P-93: Comparison of TRIS and TALP in Reversibility of The Goat Epididymal Sperm Motility Following Exposure to Different Sucrose Concentrations

Background: Nowadays, cryoprotectant-free vitrification of human sperm in large or small volumes facilitated storage and use of human sperm for long time. However, this procedure was not successful in farm animals. The main finding in the studies is higher sensitivity of these kinds of sperm to sucrose osmolarities. Preliminary studies showed a reversible effect of sucrose on goat epididymal sperm motility. However, higher concentrartions of sucrose are toxic for goat epididymal sperm. The aim of the present study is to compare recovery rate after of sucrose exposed-goat epididymal sperm in two different media (TALP or TRIS). Materials and Methods: The goat testes-epididymides were collected (4 pairs) and epididymal sperm retrieved in warmed (5 mL) basic media (TALP or TRIS (TRIS 24 mg/mL; citric acid 10 mg/mL; Glocose 8 mg/mL; genetamycine 1mg/mL and 1% (w/v) BSA; pH=6.8-7.2)) for 15 min. The sperm suspension was homogenized and an aliquot of 200 μL was overlaid with the basic media within 1.5 mL Eppendorph micro-tube and left in room temperature (25-33 C) for 30-45 minutes. The swimmedup sperm were collected from upper layer of micro-tube. Suspension of sperm adjusted with concentration of 20